Monocyte chemotactic protein-1 production in patients with active pulmonary tuberculosis and tuberculous pleurisy.

Abstract

OBJECTIVE:

The role of monocyte chemotactic protein (MCP)-1 in human pulmonary and pleural tuberculosis (TB) was assessed by examining its production in clinical samples from patients with active pulmonary TB and tuberculous pleurisy (TBP).

METHODS:

Serum was obtained from 26 active pulmonary TB patients [14 early TB (E-TB), and 12 chronic refractory TB (CR-TB)] and 15 healthy tuberculin reactors (HTRs). The monocytes and peripheral blood mononuclear cells (PBMCs) were separated and stimulated with purified protein derivatives (PPD) or the 30-kDa antigen of Mycobacterium tuberculosis. Pleural exudates were isolated from 25 patients with TBP and 24 non-TBP patients [malignancy and congestive heart failure (CHF)]. The MCP-1 levels were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS:

In sera, the MCP-1 levels of TB patients were similar to those of HTRs. For monocytes, CR-TB patients spontaneously expressed more MCP-1, compared with HTRs and E-TB patients. In addition, MCP-1 production of PPD- or 30-kDa antigen-stimulated monocytes was significantly elevated in CR-TB patients than that from E-TB. Interestingly, the E-TB patients had significantly depressed MCP-1 production by PBMCs in response to PPD or 30-kDa, compared with HTRs and CR-TB patients. In pleural effusions, MCP-1 levels were significantly higher in patients with TBP than in patients with CHF, but lower than in malignant effusions.

CONCLUSIONS:

The data suggest that MCP-1 production is not uniquely elevated systemically in TB patients, although MCP-1 production might be elevated by monocytes in the chronic phase of TB or with a local pleural infection.

• Authors: J-S Lee, C-H Song, J-H Lim, K-S Lee, H-J Kim, J-K Park, T-H Paik, S-S Jung, E-K Jo
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 52 Issue 7 Pg. 297-304 (Jun 2003) ISSN: 1023-3830 [Print] Switzerland
• PMID: 12861395 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Chemokine CCL2
• Tuberculin
• Tumor Necrosis Factor-alpha
• Interferon-gamma

Topics

• Adult
• Antigens, Bacterial (immunology)
• Chemokine CCL2 (biosynthesis)
• Exudates and Transudates (immunology)
• Female
• Humans
• Interferon-gamma (biosynthesis)
• Male
• Monocytes (metabolism)
• Mycobacterium tuberculosis (immunology)
• Neutrophils (metabolism)
• Pleura (immunology)
• Tuberculin (metabolism)
• Tuberculosis, Pleural (metabolism)
• Tuberculosis, Pulmonary (metabolism)
• Tumor Necrosis Factor-alpha (biosynthesis)