Abstract | BACKGROUND & AIMS: The components of the kinin system, including kinongens, kininogenases, and B(2) and B(1) receptors, are expressed and activated during inflammation. Here, we investigated the expression of the kinin B(2) receptor messenger RNA, kininogen and kallikrein immunoreactivity, and the ability of kinins to contract control and inflamed gallbladders in vitro. METHODS: Human gallbladders, obtained from patients undergoing cholecystectomy either for acute cholecystitis secondary to gallstone disease or during elective gastro-entero-pancreatic surgery (controls), were processed for reverse-transcription polymerase chain reaction analysis, kallikrein and kininogen immunohistochemistry, binding studies, and in vitro contractility studies. RESULTS: CONCLUSIONS: All the components of the kinin system are expressed in the human gallbladder. Bradykinin is a powerful spasmogen via B(2) receptor activation in the normal and, especially, in the inflamed human gallbladder.
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Authors | Marcello Trevisani, Silvia Amadesi, Fabien Schmidlin, Maria T Poblete, Elisabetta Bardella, Barbara Maggiore, Selena Harrison, Carlos D Figueroa, Michele Tognetto, Giuseppe Navarra, Alessandro Turini, Nigel W Bunnett, Pierangelo Geppetti, Roberto De Giorgio |
Journal | Gastroenterology
(Gastroenterology)
Vol. 125
Issue 1
Pg. 126-35
(Jul 2003)
ISSN: 0016-5085 [Print] United States |
PMID | 12851878
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adrenergic beta-Antagonists
- Antipsychotic Agents
- Benzamides
- Bradykinin Receptor Antagonists
- Cyclooxygenase Inhibitors
- Muscarinic Antagonists
- Piperidines
- Quinuclidines
- R 715
- Receptor, Bradykinin B1
- Receptor, Bradykinin B2
- Receptors, Bradykinin
- Tritium
- SR 140333
- SR 48968
- Atropine
- icatibant
- SR 142801
- Bradykinin
- Indomethacin
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Topics |
- Adrenergic beta-Antagonists
(pharmacology)
- Adult
- Aged
- Antipsychotic Agents
(pharmacology)
- Atropine
(pharmacology)
- Benzamides
(pharmacology)
- Bradykinin
(analogs & derivatives, metabolism, pharmacology)
- Bradykinin Receptor Antagonists
- Cyclooxygenase Inhibitors
(pharmacology)
- Female
- Gallbladder
(physiology)
- Gallbladder Emptying
(drug effects, physiology)
- Gene Expression
- Humans
- Immunohistochemistry
- In Vitro Techniques
- Indomethacin
(pharmacology)
- Male
- Middle Aged
- Muscarinic Antagonists
(pharmacology)
- Piperidines
(pharmacology)
- Quinuclidines
(pharmacology)
- Receptor, Bradykinin B1
- Receptor, Bradykinin B2
- Receptors, Bradykinin
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Tritium
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