Abstract |
Sesquiterpenes ( costunolide and zaluzanin D) were purified from laurel (Laurus nobilis L.) and identified by Mass, and 1H- and 13C-NMR. These sesquiterpenes displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological changes of the nucleus, including chromatin condensation were induced in the HL-60 cells treated with the sesquiterpenes. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 11.4, 47.0, and 92.5% after a 3-day-treatment with 5, 10 and 15 micro M costunolide, respectively. The same analysis showed that the hypodiploid nuclei of HL 60 cells were increased to 12.4, 28.9 and 76.7% after a 3-day-treatment with 10, 15 and 20 micro M zaluzanin D, respectively. These findings suggest that growth inhibition by costunolide and zaluzanin D of HL-60 cells results from the induction of chromatin condensation in the HL-60 cells. On the other hand, fragmentations by these compounds of DNA to oligonucleosomal-sized fragments were not observed in the HL-60 cells.
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Authors | Hiroshige Hibasami, Yayoi Yamada, Hiroyuki Moteki, Hirotaka Katsuzaki, Kunio Imai, Kazumi Yoshioka, Takashi Komiya |
Journal | International journal of molecular medicine
(Int J Mol Med)
Vol. 12
Issue 2
Pg. 147-51
(Aug 2003)
ISSN: 1107-3756 [Print] Greece |
PMID | 12851709
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Chromatin
- Sesquiterpenes
- zaluzanin-D
- costunolide
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects, genetics)
- Cell Death
(drug effects)
- Cell Division
(drug effects)
- Chromatin
(drug effects)
- Dose-Response Relationship, Drug
- HL-60 Cells
- Humans
- Laurus
(chemistry)
- Leukemia, Promyelocytic, Acute
(drug therapy, pathology)
- Magnetic Resonance Spectroscopy
- Molecular Structure
- Sesquiterpenes
(chemistry, isolation & purification, pharmacology)
- Spectrometry, Mass, Secondary Ion
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