[Primary analysis on protein maps of Helicobacter pylori strains associated with gastric carcinoma].

Abstract

OBJECTIVE:

To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.

METHODS:

The whole-cell proteins of H. pylori were separated by two-dimensional electrophoresis (2-DE). The protein maps of four H. pylori strains associated with gastric carcinoma and nine strains that isolated from patients with non-gastric carcinoma were then compared by ImageMaster 2D v3.1. MALDI-TOF mass spectrometry was performed to identify the proteins of interest. The proteins were searched by software mascot and identified by peptide fingerprint map.

RESULTS:

Three proteins seemed to be associated with gastric carcinoma including acylneuraminate cytidylyltransferase with Mowse score 79 with the sequence coverage of 32%. The other two had no unambiguous protein to match.

CONCLUSION:

Acylneuraminate cytidylyltransferase seemed to be a specific H. pylori protein associated with the presence of gastric carcinoma. Other two were novel proteins that might be associated with gastric carcinoma. However, the mechanism needs to be explored.

• Authors: Bo-qing Li, Jian-zhong Zhang, Qing-hua Zou, Li-hua He, Xiao-mei Yan
• Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (Zhonghua Liu Xing Bing Xue Za Zhi) Vol. 24 Issue 6 Pg. 439-42 (Jun 2003) ISSN: 0254-6450 [Print] China
• PMID: 12848904 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins

Topics

• Amino Acid Sequence
• Bacterial Proteins (analysis)
• Electrophoresis, Gel, Two-Dimensional
• Helicobacter pylori (chemistry)
• Humans
• Mass Spectrometry
• Molecular Sequence Data
• Stomach Neoplasms (microbiology)