Abstract |
Hydrolysis of surfactant phospholipids by secreted phospholipases A(2) ( sPLA(2)) contributes to surfactant dysfunction in acute respiratory distress syndrome. The present study demonstrates that sPLA(2)-IIA, sPLA(2)-V, and sPLA(2)-X efficiently hydrolyze surfactant phospholipids in vitro. In contrast, sPLA(2)-IIC, -IID, -IIE, and -IIF have no effect. Since purified surfactant protein A (SP-A) has been shown to inhibit sPLA(2)-IIA activity, we investigated the in vitro effect of SP-A on the other active sPLA(2) and the consequences of sPLA(2)-IIA inhibition by SP-A on surfactant phospholipid hydrolysis. SP-A inhibits sPLA(2)-X activity, but fails to interfere with that of sPLA(2)-V. Moreover, in vitro inhibition of sPLA(2)-IIA-induces surfactant phospholipid hydrolysis correlates with the concentration of SP-A in surfactant. Intratracheal administration of sPLA(2)-IIA to mice causes hydrolysis of surfactant phosphatidylglycerol. Interestingly, such hydrolysis is significantly higher for SP-A gene-targeted mice, showing the in vivo inhibitory effect of SP-A on sPLA(2)-IIA activity. Administration of sPLA(2)-IIA also induces respiratory distress, which is more pronounced in SP-A gene-targeted mice than in wild-type mice. We conclude that SP-A inhibits sPLA(2) activity, which may play a protective role by maintaining surfactant integrity during lung injury.
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Authors | Sophie Chabot, Kamen Koumanov, Gérard Lambeau, Michael H Gelb, Viviane Balloy, Michel Chignard, Jeffrey A Whitsett, Lhousseine Touqui |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 171
Issue 2
Pg. 995-1000
(Jul 15 2003)
ISSN: 0022-1767 [Print] United States |
PMID | 12847272
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Phosphatidylcholines
- Phosphatidylglycerols
- Phospholipids
- Pulmonary Surfactant-Associated Protein A
- Phospholipases A
- Group II Phospholipases A2
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Topics |
- Airway Resistance
(genetics, physiology)
- Animals
- Electrophoresis, Polyacrylamide Gel
- Group II Phospholipases A2
- Hydrolysis
- Immunoblotting
- Intubation, Intratracheal
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Phosphatidylcholines
(metabolism)
- Phosphatidylglycerols
(antagonists & inhibitors, metabolism)
- Phospholipases A
(administration & dosage, antagonists & inhibitors, metabolism, physiology)
- Phospholipids
(analysis, antagonists & inhibitors, metabolism, physiology)
- Pulmonary Surfactant-Associated Protein A
(deficiency, genetics, metabolism, physiology)
- Respiratory Distress Syndrome
(enzymology, genetics, physiopathology, prevention & control)
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