Abstract |
The antibacterial property of 7 compounds, isolated from Erythrina variegata (Leguminosae) by repeated silica gel column chromatography, against cariogenic oral bacteria was investigated. Extensive spectroscopic study revealed that all were isoflavonoids. Among them, 3,9-dihydroxy-2,10-di(gamma,gamma-dimethylallyl)-6a,11a-dehydropterocarpan ( erycristagallin) showed the highest antibacterial activity against mutans streptococci, other oral streptococci, Actinomyces and Lactobacillus species with a minimum inhibitory concentration (MIC) range of 1.56-6.25 microg/ml, followed by 3,6a-dihydroxy-9-methoxy-2,10-di(gamma,gamma-dimethylallyl)pterocarpan (erystagallinA) and 9-hydroxy-3-methoxy-2-gamma,gamma-dimethylallylpterocarpan (orientanol B) (MIC range: 3.13-12.5 microg/ml). The antibacterial effect of erycristagallin to mutans streptococci was based on a bactericidal action. Erycristagallin (6.25 microg/ml: MIC) completely inhibited incorporation of radio-labelled thymidine into Streptococcus mutans cells. Incorporation of radio-labelled glucose into bacterial cells was also strongly inhibited at MIC, and 1/2 MIC of the compound reduced the incorporation approximately by half. The findings indicate that erycristagallin has a potential as potent phytochemical agent for prevention of dental caries by inhibiting the growth of cariogenic bacteria and by interfering with incorporation of glucose responsible for production of organic acids.
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Authors | M Sato, H Tanaka, S Fujiwara, M Hirata, R Yamaguchi, H Etoh, C Tokuda |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 10
Issue 5
Pg. 427-33
( 2003)
ISSN: 0944-7113 [Print] Germany |
PMID | 12834009
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- Heterocyclic Compounds, 4 or More Rings
- Isoflavones
- erycristagallin
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Topics |
- Actinomyces
(drug effects)
- Anti-Bacterial Agents
(chemistry, isolation & purification, pharmacology)
- Dental Caries
(microbiology)
- Erythrina
(chemistry)
- Heterocyclic Compounds, 4 or More Rings
- Isoflavones
(chemistry, isolation & purification, pharmacology)
- Lactobacillus
(drug effects)
- Microbial Sensitivity Tests
- Molecular Structure
- Streptococcus
(drug effects)
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