Monitoring method for pre- and post-liver transplantation in patients with primary hyperoxaluria type I.

Abstract

Differential diagnosis of primary hyperoxaluria type I (PH1) may become difficult once end-stage renal failure and anuria have occurred. Here we describe a rapid and sensitive method to simultaneously quantify glycolate and oxalate in plasma using a stable isotope dilution and gas chromatography-mass spectrometry. The results are provided within 2 h. The linearity of the method was validated up to 200 micromol/l of these compounds and the inter-assay precision for glycolate and oxalate was 2.4 and 2.6%, respectively (n=5), when the control plasma was spiked with 50 micromol/l of glycolate and oxalate. For healthy subjects, 1.0 ml of plasma was required and 0.1 ml for the PH1 patients. Using this method, plasma levels in non-PH1 patients under hemodialysis and in healthy subjects were determined. This method proved to be useful when used for differential diagnosis of PH1 and for monitoring the plasma levels of glycolate and oxalate in two PH1 patients before and after dialysis and liver transplantation. Plasma glycolate in these patients was dramatically decreased after liver transplantation, but plasma oxalate decreased more slowly due to remobilization of the calcium oxalate stores deposited throughout the body.

• Authors: Yoshito Inoue, Hiroaki Masuyama, Hiromichi Ikawa, Hiroshi Mitsubuchi, Tomiko Kuhara
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 792 Issue 1 Pg. 89-97 (Jul 15 2003) ISSN: 1570-0232 [Print] Netherlands
• PMID: 12829001 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adult
• Case-Control Studies
• Female
• Gas Chromatography-Mass Spectrometry (methods)
• Humans
• Hyperoxaluria, Primary (blood, surgery)
• Liver Transplantation
• Male
• Monitoring, Physiologic (methods)
• Reproducibility of Results