Abstract | BACKGROUND:
Lectins, carbohydrate proteins, bind non-covalently to glycoconjugate of normal and malignant cells. If used in cell culture, they can influence cellular proliferation. In this study the in vitro effects of six dietary lectins on the cell proliferation of human breast cancer cell lines were investigated. MATERIALS AND METHODS: Cell proliferation was assessed by a colorimetric XTT-based assay kit. Lectin binding was characterized by lectin histochemistry. RESULTS: WGA considerably influenced the cell growth of all tested cell lines (MCF-7, T 47D, HBL 100, BT 20), whereas the effects of PHA-L, SBA and HPA were smaller, began at higher concentrations and were restricted to three cell lines (MCF-7, T 47D and HBL 100 for PHA-L; MCF-7, T 47D and BT 20 for SBA, respectively) and to one cell line (HBL 100 for HPA). STA and PNA had no effect at all. CONCLUSION:
|
Authors | Ursula Valentiner, Stefanie Fabian, Udo Schumacher, Anthony J Leathem |
Journal | Anticancer research
(Anticancer Res)
2003 Mar-Apr
Vol. 23
Issue 2B
Pg. 1197-206
ISSN: 0250-7005 [Print] Greece |
PMID | 12820371
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
- Helix lectin
- Lectins
- Peanut Agglutinin
- Phytohemagglutinins
- Plant Lectins
- Soybean Proteins
- Wheat Germ Agglutinins
- potato lectin
- soybean lectin
|
Topics |
- Adenocarcinoma
(pathology)
- Breast
(cytology)
- Breast Neoplasms
(pathology)
- Carbohydrate Metabolism
- Cell Division
(drug effects)
- Cells, Cultured
(cytology, drug effects)
- Diet
- Epithelial Cells
(cytology, drug effects)
- Female
- Humans
- Lectins
(pharmacology)
- Peanut Agglutinin
(pharmacology)
- Phytohemagglutinins
(pharmacology)
- Plant Lectins
(pharmacology)
- Soybean Proteins
(pharmacology)
- Tissue Fixation
(methods)
- Tumor Cells, Cultured
(cytology, drug effects)
- Wheat Germ Agglutinins
(pharmacology)
|