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Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids.

Abstract
Sequence analysis of the Ogura-specific mitochondrial DNA (mtDNA) fragment isolated previously from Brassica cybrids carrying Ogura cytoplasmic male sterility (cms) revealed a tRNA(fMet) sequence, a putative 138 amino acid open reading frame (orf138), and a 158 amino acid ORF (orf158) previously observed in mitochondrial genomes from several other plant species. Transcription mapping showed that both ORFs are present on a 1.4 kb cms-specific transcript. The orf158 sequence is also transcribed in fertile plants on a different mRNA, and thus is unlikely to be related to cms. On the other hand, fertile revertant plants lack transcripts of the orf138 sequence, whose possible role in the mechanism of Ogura cms is discussed.
AuthorsS Bonhomme, F Budar, D Lancelin, I Small, M C Defrance, G Pelletier
JournalMolecular & general genetics : MGG (Mol Gen Genet) Vol. 235 Issue 2-3 Pg. 340-8 (Nov 1992) ISSN: 0026-8925 [Print] Germany
PMID1281515 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • DNA, Mitochondrial
  • RNA, Messenger
  • RNA, Transfer, Amino Acyl
  • RNA, Transfer, Met
  • tRNA, formylmethionine-
  • RNA
  • endodeoxyribonuclease NcoI
  • Deoxyribonucleases, Type II Site-Specific
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Brassica (genetics, physiology)
  • Cloning, Molecular
  • DNA, Mitochondrial (genetics, isolation & purification)
  • Deoxyribonucleases, Type II Site-Specific
  • Fertility (genetics)
  • Genotype
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA (genetics, isolation & purification)
  • RNA, Messenger (genetics)
  • RNA, Transfer, Amino Acyl (genetics)
  • RNA, Transfer, Met
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

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