Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin ).
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| Abstract |
Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin ), produced by Biobr s (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30 g of each protein at 15-day intervals combined with 250 microg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-gamma induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.
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| Authors | Sandra Regina Afonso Cardoso, João Carlos França da Silva, Roberto Teodoro da Costa, Wilson Mayrink, Maria Norma Melo, Marilene Suzan Marques Michalick, Ibrahim Afrânio Willi Liu, Ricardo Toshio Fujiwara, Evaldo Nascimento |
| Journal | Revista da Sociedade Brasileira de Medicina Tropical (Rev Soc Bras Med Trop) 2003 Mar-Apr Vol. 36 Issue 2 Pg. 193-9 ISSN: 0037-8682 [Print] Brazil |
| PMID | 12806454 (Publication Type: Journal Article) |
| Chemical References |
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