DNA vaccination protects against botulinum neurotoxin type F.

Abstract

A DNA vaccine was constructed which expressed the binding domain of Clostridium botulinum neurotoxin serotype F fused to a signal peptide. Three intra-muscular doses fully protected Balb/c mice against 10(4) MLD of serotype F toxin. Priming of the immune response by DNA vaccination followed by a single booster with type F binding domain protein resulted in high levels of antibody against the binding domain. This study demonstrates the utility of DNA vaccination for protection against botulinum neurotoxin type F and indicates that a prime-boost regimen could be an efficient method of generating antibody for passive immune therapy in cases of botulism involving serotype F toxin.

• Authors: Alice M Bennett, Stuart D Perkins, Jane L Holley
• Journal: Vaccine (Vaccine) Vol. 21 Issue 23 Pg. 3110-7 (Jul 04 2003) ISSN: 0264-410X [Print] Netherlands
• PMID: 12804837 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Immunoglobulin G
• Immunoglobulins
• Vaccines, DNA
• Botulinum Toxins
• botulinum toxin type F

Topics

• Animals
• Antibodies, Bacterial (biosynthesis)
• Botulinum Toxins (immunology, toxicity)
• COS Cells
• Chlorocebus aethiops
• Enzyme-Linked Immunosorbent Assay
• Female
• Fluorescent Antibody Technique
• Immunoglobulin G (biosynthesis, immunology)
• Immunoglobulins (biosynthesis, immunology)
• Injections, Intramuscular
• Mice
• Mice, Inbred BALB C
• Microscopy, Confocal
• Plasmids (genetics)
• Survival Analysis
• Vaccination
• Vaccines, DNA (biosynthesis, immunology)