RP 58802B, a long-acting beta 2-adrenoceptor agonist: assessment of antiasthma activity in the guinea-pig in vivo.

Abstract

We have examined the protective actions of RP 58802B, a novel beta 2-adrenoceptor agonist, administered by the inhaled and oral routes in the anaesthetized and conscious guinea-pig against bronchospasm induced by histamine or antigen (ovalbumin). We have also examined the effects of RP 58802B on airway reactivity and inflammatory cell infiltration in platelet-activating factor (PAF) (aerosol)-induced bronchial hyperreactivity and on PAF (tracheal instillation)-induced microvascular leakage in the guinea-pig. Nebulized RP 58802B produced a rapid onset and long lasting inhibition of histamine-induced bronchospasm in the anaesthetized guinea-pig (EC50 = 3.2 +/- 0.9 micrograms/ml; duration greater than 90 min). Given orally, RP 58802B (5 mg/kg, 60 min before challenge) produced a greater than three-fold shift to the right of the dose-response curve and depressed the maximum response to histamine by 39 +/- 11%. Increasing the concentration to 25 mg/kg had no futher effect. Similar protection was still seen 4 h after oral dosing. In conscious guinea-pigs, RP 58802B (5 or 25 mg/kg, p.o. 60 min before challenge) significantly attenuated antigen-induced dyspnoea with the time to severe dyspnoea increasing from 170 +/- 32 to 325 +/- 32 s at the higher dose of drug. RP 58802B (10 or 25 mg/kg, p.o. 60 min before exposure to PAF) prevented the development of bronchial hyperreactivity. Although PAF-induced bronchial hyperreactivity was not accompanied by an increase in the number of pulmonary eosinophils, RP 58802B (25 mg/kg p.o.) reduced the numbers of eosinophils recovered by lavage. RP 58802B (10 mg/kg p.o.) significantly inhibited PAF-induced microvascular leakage into guinea-pig lung. These data suggest that RP 58802B, in addition to being a potent and long acting bronchodilator, may have a prophylactic role in preventing bronchial hyperreactivity and in reducing plasma exudation into the lungs.

• Authors: S L Underwood, S A Lewis, D Raeburn
• Journal: Pulmonary pharmacology (Pulm Pharmacol) Vol. 5 Issue 3 Pg. 203-12 (Sep 1992) ISSN: 0952-0600 [Print] England
• PMID: 1280178 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adrenergic beta-Agonists
• Antigens
• Benzimidazoles
• Dextrans
• Ethanolamines
• Platelet Activating Factor
• fluorescein isothiocyanate dextran
• Histamine
• RP 58802B
• Fluorescein-5-isothiocyanate
• Albuterol

Topics

• Administration, Inhalation
• Administration, Oral
• Adrenergic beta-Agonists (pharmacology)
• Albuterol (pharmacology)
• Anesthesia
• Animals
• Antigens (administration & dosage)
• Asthma (drug therapy, physiopathology)
• Benzimidazoles (pharmacology)
• Bronchi (drug effects, metabolism)
• Bronchial Hyperreactivity (chemically induced, drug therapy)
• Bronchoalveolar Lavage Fluid (metabolism)
• Bronchoconstriction (drug effects, physiology)
• Dextrans (pharmacokinetics)
• Ethanolamines (pharmacology)
• Fluorescein-5-isothiocyanate (pharmacokinetics)
• Guinea Pigs
• Histamine (pharmacology)
• Male
• Platelet Activating Factor (pharmacology)
• Time Factors
• Trachea (drug effects, metabolism)