HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Flucytosine and cryptococcosis: which in vitro test is the best predictor of outcome?

Abstract
The combination of flucytosine and amphotericin B is first choice treatment for active cryptococcosis. Because of innate or acquired resistance of Cryptococcus neoformans to flucytosine, in vitro testing is mandatory. Yeast nitrogen base (YNB) at pH 7.0 is the recommended medium for the broth microdilution test (NCCLS M27-A) and for the E-test. In order to verify if minimum inhibitory concentrations (MICs) were able to predict treatment outcome, the susceptibility of 24 isolates from 21 patients treated with flucytosine alone or in combination was tested by the broth microdilution, agar dilution and E-test using YNB either at pH 7.0 or at pH 5.4. Only those MICs obtained on YNB pH 5.4 proved to correlate with treatment outcome. The present study suggests that in vitro susceptibility to flucytosine of C. neoformans isolates should be evaluated on YNB pH 5.4 and the test should be standardized accordingly.
AuthorsM A Viviani, M C Esposto, M Cogliati, A M Tortorano
JournalJournal of chemotherapy (Florence, Italy) (J Chemother) Vol. 15 Issue 2 Pg. 124-8 (Apr 2003) ISSN: 1120-009X [Print] England
PMID12797387 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Bacterial Agents
  • Antifungal Agents
  • Culture Media
  • Amphotericin B
  • Flucytosine
Topics
  • Adult
  • Amphotericin B (administration & dosage, pharmacology)
  • Anti-Bacterial Agents (administration & dosage, pharmacology)
  • Antifungal Agents (administration & dosage, pharmacology)
  • Cryptococcosis (drug therapy)
  • Cryptococcus neoformans (drug effects, isolation & purification, pathogenicity)
  • Culture Media
  • Drug Therapy, Combination
  • Female
  • Flucytosine (administration & dosage, pharmacology)
  • Humans
  • Hydrogen-Ion Concentration
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Predictive Value of Tests
  • Treatment Outcome

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: