PH-027 is a new 5-triazole
oxazolidinone synthesized in our laboratories, which shows strong activity against gram-positive aerobic bacteria including clinical isolates. The objective of this study was to investigate the in vitro activity of this compound in comparison with
linezolid and other
antibiotics against gram-positive and gram-negative anaerobes. The in vitro activity of
PH-027 in comparison with those of
linezolid and other
antimicrobial agents was evaluated against 201 clinical isolates of gram-positive and gram-negative anaerobic bacteria by
agar dilution and Etest methods.
PH-027 showed excellent activity, with minimum inhibitory concentrations (MIC) in the range of 0.12-4.0 microg/ml against all isolates; MIC90s being 4.0, 1.0, 2.0, 2.0 and 2.0 microg/ml against Clostridium difficile, Peptostreptococcus spp., Bacteroides fragilis, Prevotella bivia and Fusobacterium spp. respectively. In comparison,
linezolid had MIC in the range of 0.5-4.0 microg/ml against all isolates, with MIC90s of 2.0, 4.0, 4.0, 4.0 and 2.0 microg/ml against the same set of bacteria respectively.
PH-027 demonstrated excellent in vitro activity that is superior to
linezolid against Peptostreptococcus spp., B. fragilis and P. bivia. However, against C. difficile and Fusobacterium spp,
PH-027 and
linezolid showed comparable in vitro activity. Against all anaerobes,
metronidazole,
PH-027 and, to a lesser extent,
linezolid had the most potent activity. From the results of in vitro susceptibility testing, both
linezolid and
PH-027 show promise in the treatment of anaerobic
infections.