Clinical usefulness of edaravone for acute liver injury.

Abstract

BACKGROUND:

Edaravone, a newly synthesized radical scavenger, has shown an excellent effect on treating stroke patients. The effect of edaravone on carbon tetrachloride (CCl4)-induced acute liver injury was examined.

METHODS:

Six rats were injected with CCl4 alone and six rats were intravenously injected with edaravone immediately after and 3 h after injection of CCl4. Another six rats were injected with olive oil alone. The animals were killed at 24 h after the CCl4 injection.

RESULTS:

Injection of CCl4 was followed by a marked increase in serum alanine aminotranferase (ALT) level (CCl4, 1630.6 +/- 606.8 IU/L; olive oil, 21.0 +/- 2.6 IU/L; P < 0.001), lactate dehydrogenase (LDH) level (CCl4, 5068.0 +/- 2956.4 IU/L; olive oil, 203.6 +/- 30.5 IU/L; P < 0.005), and total bilirubin (TB) level (CCl4, 0.88 +/- 0.48 mg/dL; olive oil, 0.37 +/- 0.05 mg/dL; P < 0.01), whereas in the edaravone-treated rats, the ALT (119.4 +/- 113.5 IU/L, P < 0.001), LDH (369.7 +/- 288.2 IU/L, P < 0.005), and TB values (0.29 +/- 0.16 mg/dL, P < 0.01) were significantly decreased. Histological examination of the liver by hematoxylin and eosin and oil red O staining showed a marked reduction of steatosis in the CCl4 and edaravone-treated rats compared with the CCl4-injected rats. Significant inhibition of hepatocytic apoptosis was demonstrated by the terminal deoxynucleotidyl transferase-mediated UTP nick-end labeling (TUNEL) method in the edaravone-treated rats.

CONCLUSIONS:

These results suggest that edaravone has a marked preventive effect on oxidative stress-induced acute liver injury.

• Authors: Shinchiro Tada, Nobuhiro Nakamoto, Kaori Kameyama, Satoshi Tsunematsu, Naoki Kumagai, Hidetsugu Saito, Hiromasa Ishii
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 18 Issue 7 Pg. 851-7 (Jul 2003) ISSN: 0815-9319 [Print] Australia
• PMID: 12795759 (Publication Type: Journal Article)
• Copyright: Copyright 2003 Blackwell Publishing Asia Pty Ltd

Chemical References

• Free Radical Scavengers
• Carbon Tetrachloride
• Edaravone
• Antipyrine

Topics

• Animals
• Antipyrine (analogs & derivatives, pharmacology)
• Apoptosis (drug effects)
• Carbon Tetrachloride
• Chemical and Drug Induced Liver Injury
• Edaravone
• Free Radical Scavengers (pharmacology)
• Liver Diseases (prevention & control)
• Liver Function Tests
• Male
• Rats
• Rats, Wistar
• Statistics, Nonparametric