Proteomic profiling of pancreatic ductal carcinoma cell lines treated with trichostatin-A.

Abstract

A pancreatic adenocarcinoma cell line (Paca44) was treated with trichostatin-A (TSA), a potent inhibitor of histone deacetylases, in order to evaluate the effect of this drug on protein expression. Master maps of control and treated Paca44 cells were generated by analysis with the PDQuest software. The comparison between such maps showed up- and downregulation of 51 polypeptide chains, out of a total of 700 spots detected by a medium-sensitivity stain, micellar Coomassie Brilliant Blue. Fingerprinting by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF)-mass spectrometry analysis enabled the identification of 22 of these spots. Among these proteins, of particular interest are the two downregulated proteins nucleophosmin and translationally controlled tumor protein, as well as the upregulated proteins programmed cell death protein 5 (also designated as TFAR19) and stathmin (oncoprotein 18). The modulation of these four proteins is consistent with our observation that TSA is able to inhibit cell growth of Paca44 by causing cell cycle arrest at the G2 phase and apoptotic cell death.

• Authors: Daniela Cecconi, Aldo Scarpa, Massimo Donadelli, Marta Palmieri, Mahmoud Hamdan, Hubert Astner, Pier Giorgio Righetti
• Journal: Electrophoresis (Electrophoresis) Vol. 24 Issue 11 Pg. 1871-8 (Jun 2003) ISSN: 0173-0835 [Print] Germany
• PMID: 12783462 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Histone Deacetylase Inhibitors
• Hydroxamic Acids
• Proteins
• trichostatin A

Topics

• Antineoplastic Agents (pharmacology, therapeutic use)
• Carcinoma, Pancreatic Ductal (chemistry, drug therapy, pathology)
• Cell Division (drug effects)
• Cell Line, Tumor
• Electrophoresis, Gel, Two-Dimensional (methods)
• Gene Expression Regulation, Neoplastic
• Histone Deacetylase Inhibitors
• Humans
• Hydroxamic Acids (pharmacology, therapeutic use)
• Proteins (analysis)
• Proteomics (methods)
• Software
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)