Respiratory syncytial virus-induced CCL5/RANTES contributes to exacerbation of allergic airway inflammation.

Abstract

Severe respiratory syncytial virus (RSV) infection has a significant impact on airway function and may induce or exacerbate the response to a subsequent allergic challenge. In a murine model combining early RSV infection with later cockroach allergen (CRA) challenge, we examined the role of RSV-induced CCL5/RANTES production on allergic airway responses. RSV infection increased CCL5 mRNA and protein levels, peaking at days 8 and 12, respectively. Administration of CCL5 antiserum during days 0-14 of the RSV infection did not significantly alter viral protein expression when compared to mice treated with control serum. In mice receiving the combined RSV-allergen challenge, lungs collected on day 22 exhibited significantly increased numbers of CD4- and CD8-positive T cells. This increase in T cell numbers was not observed in mice receiving alpha-CCL5. On day 43, peribronchial eosinophilia and leukotriene levels were increased in RSV-allergen mice. Pretreatment with CCL5 antiserum resulted in decreased recruitment of inflammatory cells to bronchoalveolar and peribronchial regions of the lungs and these reductions were associated with a reduction in both T cell recruitment into the bronchoalveolar space, leukotriene release and chemokine generation. Thus, CCL5 released during RSV infection has a significant effect on the inflammatory response to subsequent allergic airway challenges.

• Authors: Alison E John, Aaron A Berlin, Nicholas W Lukacs
• Journal: European journal of immunology (Eur J Immunol) Vol. 33 Issue 6 Pg. 1677-85 (Jun 2003) ISSN: 0014-2980 [Print] Germany
• PMID: 12778486 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Allergens
• Chemokine CCL5
• Chemokines
• Immune Sera
• Leukotrienes
• Viral Proteins

Topics

• Allergens (immunology)
• Animals
• Asthma (etiology)
• Bronchi (immunology, pathology)
• Bronchoalveolar Lavage Fluid (immunology)
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Chemokine CCL5 (antagonists & inhibitors, biosynthesis, immunology, physiology)
• Chemokines (biosynthesis)
• Chemotaxis, Leukocyte
• Cockroaches (immunology)
• Eosinophilia (etiology)
• Female
• Flow Cytometry
• Gene Expression Regulation, Viral
• Humans
• Immune Sera
• Inflammation
• Leukotrienes (metabolism)
• Lung (immunology, pathology)
• Mice
• Mice, Inbred DBA
• Models, Animal
• Respiratory Hypersensitivity (etiology, physiopathology, prevention & control)
• Respiratory Syncytial Virus Infections (complications, immunology, physiopathology)
• Specific Pathogen-Free Organisms
• Viral Proteins (biosynthesis)