A galactosidase immunosorbent test for carcinoembryonic antigen.

A galactosidase immunosorbent test for carcinoembryonic antigen (CEA) is described in which the amount of galactosidase adsorbed to a cellulose disc is a hyperbolic function of CEA concentration. Thus, molecules with CEA-like activity can be characterized by mathematical analysis of data obtained from the galactosidase immunosorbent test. By such analysis, CEA-reactive molecules in normal human plasma were distinguished from normal cross-reacting antigen and from authentic CEA. Variation of the amount of antibody-enzyme conjugate used in the galactosidase immunosorbent test permitted CEA-reactive material in plasma of a patient with rectal carcinoma to be antigenically distinguished from the CEA-reactive material in urine of a patient with bladder carcinoma. The galactosidase immunosorbent test is a useful tool for analysis of CEA-reactive molecules.
AuthorsA R Frackelton Jr, R P Szaro, J K Weltman
JournalCancer research (Cancer Res) Vol. 36 Issue 8 Pg. 2845-9 (Aug 1976) ISSN: 0008-5472 [Print] UNITED STATES
PMID1277194 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Carcinoembryonic Antigen
  • Galactosidases
  • Absorption
  • Antibody Specificity
  • Carcinoembryonic Antigen (analysis)
  • Galactosidases
  • Humans
  • Immunoassay (methods)

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