Effects of Daflon on oxidative stress induced by hindlimb ischemia/reperfusion.

Abstract

The objective of this study was to investigate the effects of Daflon 500 mg on tissue damage in kidney after ischemia/reperfusion hindlimb, by assessing blood biochemical assay and histopathological analysis. Rats were given Daflon 80 mg x kg(-1) x day(-1) for 10 days. On 11th day of treatment, 4h ischemia followed by 4 h reperfusion period was performed on right hind limb of the rats. Control groups were given only arabic gum and were subjected to same ischemia/reperfusion period. At the end of reperfusion period, erythrocyte superoxide dismutase, Na(+)-K(+) ATPase and reduced glutathione levels were increased in the rats erythrocytes in Daflon group (P<0.01, for all). On the other hand, serum myeloperoxidase and malondialdehyde levels were significantly lower in the Daflon-received rats (P<0.01, for all). Histopathological studies demonstrated that, there was a prominent tubulointerstitial injury with loss of brush border and this degeneration was accompanied by segmental glomerular degeneration also for both control and Daflon group. Daflon-received group animals displayed significantly less periglomerular and perivascular leukocytic infiltration (P=0.015). These overall results suggest that Daflon contributes renal protection from hind limb ischemia/reperfusion injury in some degree, by decreasing systemic oxidative stress.

• Authors: A Unlü, N Sucu, L Tamer, B Coskun, G Yücebilgiç, B Ercan, A Gül, M Dikmengil, U Atik
• Journal: Pharmacological research (Pharmacol Res) Vol. 48 Issue 1 Pg. 11-5 (Jul 2003) ISSN: 1043-6618 [Print] Netherlands
• PMID: 12770509 (Publication Type: Journal Article)

Chemical References

• Protective Agents
• Malondialdehyde
• Diosmin
• Peroxidase
• Superoxide Dismutase
• Sodium-Potassium-Exchanging ATPase
• Glutathione

Topics

• Animals
• Diosmin (pharmacology, therapeutic use)
• Glutathione (metabolism)
• Hindlimb
• Kidney (drug effects, metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Nephritis, Interstitial (chemically induced, physiopathology)
• Oxidative Stress (drug effects, physiology)
• Peroxidase (metabolism)
• Protective Agents (pharmacology, therapeutic use)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, metabolism)
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Superoxide Dismutase (metabolism)