Abstract | BACKGROUND: METHODS: Rat ileitis was induced by the intraluminal injection of a TNBS solution (160 mg/kg in 50% ethanol) at a site 10 cm proximal to the ileocecal valve. The rats then received oral IS-741 (50 mg/kg) or saline for 7 days. On the day 8 after the initial administration of IS-741 or saline, we determined the visible damage score, and assessed myeloperoxidase (MPO) activity. Concentrations of cytokines in the ileum, such as interleukin-8 (IL-8) and tumor necrosis factor-alpha ( TNF-alpha) were assayed by enzyme-linked immunosorbent assay (ELISA). We also investigated the infiltration of polymorphonuclear cells and Mac-1 positive cells by histological examinations. RESULTS: The administration of IS-741 resulted in a significant reduction of the visible damage score, myeloperoxidase (MPO) activity, and mucosal IL-8 levels in the ileum as compared with the saline administration. IS-741 also dramatically reduced the infiltration of polymorphonuclear cells and Mac-1 positive cells into the inflamed lesions. CONCLUSIONS:
|
Authors | Tetsuya Fukunaga, Tomoyuki Tsujikawa, Masaya Sasaki, Yoshihide Fujiyama, Tadao Bamba, Mineko Fujimiya |
Journal | Journal of gastroenterology
(J Gastroenterol)
Vol. 38
Issue 5
Pg. 451-9
( 2003)
ISSN: 0944-1174 [Print] Japan |
PMID | 12768387
(Publication Type: Journal Article)
|
Chemical References |
- Pyridines
- IS 741
- Trinitrobenzenesulfonic Acid
- Peroxidase
- Phospholipases A
|
Topics |
- Animals
- Disease Models, Animal
- Ileitis
(chemically induced, drug therapy, physiopathology)
- Immunohistochemistry
- Male
- Neutrophil Infiltration
(drug effects, physiology)
- Peroxidase
(metabolism)
- Phospholipases A
(antagonists & inhibitors)
- Pyridines
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Trinitrobenzenesulfonic Acid
(pharmacology)
|