Abstract |
Immunization of mice with in vitro reconstituted alpha2-macroglobulin-peptide complexes primes peptide-specific CTL responses. We show here using the H-Y antigenic system that naturally produced, immunogenic alpha2-macroglobulin-peptide complexes can be isolated from the sera of normal male mice. As an application of these ideas to cancer immunity, we show that the immunity evoked by alpha2-macroglobulin-peptide complexes reconstituted in vitro is effective in prophylaxis against tumors. Furthermore, complex peptide mixtures isolated from tumor lysates can be reconstituted non-covalently with alpha2-macroglobulin and such complexes elicit potent protective tumor immunity. This approach circumvents the need for prior knowledge of the identity of the immunogenic peptides. The heat shock protein/ alpha2-macroglobulin receptor CD91 is shown to be involved in the ability of heat shock proteins or alpha2-macroglobulin to elicit an anti- tumor immune response.
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Authors | Robert J Binder, Sumeet K Kumar, Pramod K Srivastava |
Journal | Cancer immunity
(Cancer Immun)
Vol. 2
Pg. 16
(Dec 18 2002)
ISSN: 1424-9634 [Electronic] United States |
PMID | 12747761
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Heat-Shock Proteins
- Low Density Lipoprotein Receptor-Related Protein-1
- Peptides
- alpha-Macroglobulins
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Topics |
- Animals
- Heat-Shock Proteins
(metabolism)
- Immunity, Cellular
- Immunization
- Immunotherapy
- Low Density Lipoprotein Receptor-Related Protein-1
(immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Neoplasms, Experimental
(prevention & control)
- Peptides
(immunology, metabolism)
- T-Lymphocytes, Cytotoxic
(immunology)
- alpha-Macroglobulins
(immunology, metabolism)
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