Abstract | BACKGROUND: METHODS: RESULTS: The transcripts for the three markers were highly expressed in the secretory epithelium of normal lateral prostate (LP). Their hybridization signals became reduced in the epithelial cells in the low-grade PINs and significantly weakened or lost in the high-grade PINs induced in the LP. Interestingly, we observed that some dysplastic cells located at the basal compartment of the PIN lesions, and nests of outpouching epithelial cells in the vicinity of PINs, expressed positive hybridization signals of three markers. In the adenocarcinoma, signals of probasin but not PSP94 and SVSII were detected. No hybridization signals were detected in both Dunning and AIT tumors. By RT-PCR, transcripts for these proteins were still detected but significantly reduced in the Dunning tumors, whereas in the AIT tumor, only SVSII transcripts were detected. Immunohistochemistry of PSP94 also showed a reduced staining in the PIN lesions, but no immunoreactivity was seen in the rat prostatic tumors. CONCLUSIONS: The mRNA expression of the three prostatic secretory markers were decreased in the hormone-induced PINs and in two rat prostatic tumors, indicating that the androgen-regulated secretory differentiation was impaired during the development of the premalignant lesion and further reduced in advanced tumors. The abnormal expression pattern of these secretory markers and androgen receptor (AR) in the basal compartment of the PIN lesions suggests that there is a population of cell types with secretory phenotype appearing in the basal cell layer during the early malignant transformation of the prostatic epithelium.
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Authors | Joseph Kwong, K Lui, Peter S F Chan, Shuk-Mei Ho, Y C Wong, J W Xuan, Franky L Chan |
Journal | The Prostate
(Prostate)
Vol. 56
Issue 2
Pg. 81-97
(Jul 01 2003)
ISSN: 0270-4137 [Print] United States |
PMID | 12746832
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2003 Wiley-Liss, Inc. |
Chemical References |
- Androgen-Binding Protein
- Biomarkers, Tumor
- Prostatic Secretory Proteins
- RNA, Messenger
- Semg1 protein, rat
- Seminal Vesicle Secretory Proteins
- beta-microseminoprotein
- probasin
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Topics |
- Adenocarcinoma
(physiopathology)
- Androgen-Binding Protein
(analysis, genetics)
- Animals
- Biomarkers, Tumor
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
- Immunohistochemistry
- In Situ Hybridization
- Male
- Prostate
(chemistry, metabolism, physiopathology)
- Prostatic Intraepithelial Neoplasia
(chemistry, physiopathology)
- Prostatic Neoplasms
(chemistry, physiopathology)
- Prostatic Secretory Proteins
(analysis, genetics)
- RNA, Messenger
(analysis)
- Rats
- Rats, Inbred Strains
- Reverse Transcriptase Polymerase Chain Reaction
- Seminal Vesicle Secretory Proteins
(analysis, genetics)
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