Abstract |
An identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (PCR-RFLP) was developed to differentiate isolates of the genera Campylobacter, Arcobacter and Helicobacter. Based on the 16S rRNA gene of these genera, PCR amplified a 1216-bp fragment. The amplicons were digested with the restriction enzymes RsaI and EcoRV. Additional differentiation was obtained using a PCR-assay based on the hippuricase gene. Genotyping was performed on several reference strains from the National Collection of Typing Culture (NCTC), London, and on 130 field isolates. In parallel, a phenotypic differentiation was performed, in order to compare the results. In 119 cases (91.5%) the results obtained from the genotypic characterization were concordant with those from phenotypic testing. Co-infections with Campylobacter jejuni and Campylobacter coli in two samples and seven hippurate-negative C. jejuni-strains were identified by the genotypic method. Furthermore, PCR-RFLP assays identified an atypical isolate as Campylobacter fetus/hyointestinalis.
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Authors | V Jauk, C Neubauer, W Szölgyényi, L Vasicek |
Journal | Avian pathology : journal of the W.V.P.A
(Avian Pathol)
Vol. 32
Issue 1
Pg. 33-7
(Feb 2003)
ISSN: 0307-9457 [Print] England |
PMID | 12745378
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
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Topics |
- Animals
- Austria
(epidemiology)
- Campylobacter
(genetics, isolation & purification)
- Campylobacter Infections
(epidemiology, microbiology, veterinary)
- Chickens
- Genotype
- Phenotype
- Polymorphism, Restriction Fragment Length
- Poultry Diseases
(epidemiology, microbiology)
- RNA, Ribosomal, 16S
(genetics)
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