Abstract | OBJECTIVE: METHODS: The dogs were classified into five groups to identify the injury of myocardium and coronary endothelium: group 1, normothermic CPB without cardiac arrest; group 2, hypothermic CPB with continuous tepid blood cardioplegia, and with cardiac arrest; group 3, hypothermic CPB with intermittent cold blood cardioplegia, and with cardiac arrest; group 4, hypothermic CPB with intermittent cold crystalloid cardioplegia, and with cardiac arrest; and group 5, sham-operated control group. During CPB, cardiac arrest was achieved with different cardioplegia solutions for 60 min, followed by reperfusion for 4 h before the myocardium and coronary arteries were harvested. Coronary arteries were harvested immediately and analyzed by scanning electron microscopy. Cardiomyocytic apoptosis was detected using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate- biotin nick end labeling, Western blot, and DNA ladder methods. RESULTS: Regardless of the detection method used, significantly higher percentages of apoptotic cardiomyocytes were found in group 3 and group 4 than in other groups. Expression of caspase-3 correlated with increased apoptosis. Scanning electron microscopy revealed severe endothelial injury of coronary arteries in group 3 and group 4. CONCLUSION: These results point to an important explanation for the difference in cardiac recovery after hypothermic ischemia and arrest with various cardioplegic solutions.
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Authors | Chi-Hsiao Yeh, Yao-Chang Wang, Yi-Cheng Wu, Jaw-Ji Chu, Pyng Jing Lin |
Journal | Chest
(Chest)
Vol. 123
Issue 5
Pg. 1647-54
(May 2003)
ISSN: 0012-3692 [Print] United States |
PMID | 12740286
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Apoptosis
- Blood
- Blotting, Western
- Cardioplegic Solutions
(administration & dosage)
- Cardiopulmonary Bypass
- Coronary Vessels
(ultrastructure)
- Dogs
- Endothelium, Vascular
(ultrastructure)
- Heart Arrest, Induced
(methods)
- In Situ Nick-End Labeling
- Microscopy, Electron, Scanning
- Myocardial Reperfusion Injury
(pathology, prevention & control)
- Myocardium
(ultrastructure)
- Temperature
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