Abstract | PURPOSE: EXPERIMENTAL DESIGN: We examined the expression of 4N1K-containing proteins and TSP1, microvessel density, proliferation index, and apoptotic index in 119 surgically excised RCC tissues by immunohistochemical techniques. The correlation between the above variables and clinicopathological features was analyzed statistically. RESULTS: The antibody raised against the 4N1K peptide recognized protein fragments of matrix metalloproteinase 3-digested TSP1 and positively stained the sections of renal cancer tissues. These reactions disappeared when the antibody was preincubated with immobilized 4N1K peptide, suggesting that the reactions were 4N1K peptide specific. Although TSP1 expression did not correlate with various clinicopathological features and tumor size, all 4N1K-positive tumors were locally confined and of significantly smaller size (median, 3.3 cm; range, 2.0-4.4 cm) than 4N1K-negative tumors (median, 5.2 cm; range, 2.8-8.8 cm; P < 0.001). 4N1K-positive tumors exhibited significantly lower microvessel density and higher apoptotic index of tumor cells than 4N1K-negative tumors (P < 0.001 and P = 0.042, respectively). CONCLUSIONS: Our data suggest that expression of 4N1K-containing proteins in tumor tissues is associated with reduced angiogenesis and tumor growth; thus, it would be a potentially predictive marker for progression of RCC.
|
Authors | Yasuyoshi Miyata, Shigehiko Koga, Kousuke Takehara, Hiroshi Kanetake, Shigeru Kanda |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 9
Issue 5
Pg. 1734-40
(May 2003)
ISSN: 1078-0432 [Print] United States |
PMID | 12738728
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- 4N1K peptide
- Biomarkers, Tumor
- Oligopeptides
- Thrombospondin 1
|
Topics |
- Adult
- Aged
- Apoptosis
- Biomarkers, Tumor
(metabolism)
- Carcinoma, Renal Cell
(blood supply, metabolism, pathology)
- Cell Division
- Female
- Humans
- Kidney Neoplasms
(blood supply, metabolism, pathology)
- Male
- Middle Aged
- Neovascularization, Pathologic
(metabolism)
- Oligopeptides
(metabolism)
- Thrombospondin 1
(metabolism)
|