Abstract |
In the last decades, substances with estrogenic activity have been dispersed into the environment. Xenoestrogens act by binding to estrogen receptors, ligand-regulated transcription factors, for which two subtypes have been described, ER-alpha and ER-beta, which are often coexpressed at variable amounts in different tissues. We investigated variations in the expression of ER-alpha and ER-beta mRNAs following treatment with four xenoestrogens ( bisphenol A, 4-tert octylphenol, 2-hydroxybiphenyl, 4-hydroxybiphenyl) and with 17beta-estradiol in estrogen-sensitive (T47D) and estrogen-insensitive (BT20) breast cancer cell lines. Although to a variable extent, both estradiol and the tested xenoestrogens increased the expression of ER-beta mRNA, whereas a slight effect on ER-alpha was observed only in T47D cells. Upregulation of ER-beta expression by estradiol and xenoestrogens was observed only in the presence of detectable ER-alpha protein levels. These findings indicate a regulatory role for ER-beta in ER-alpha-mediated transcription and a role for ER-beta in mediating xenoestrogen toxicity.
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Authors | V Cappelletti, G Saturno, P Miodini, W Körner, M G Daidone |
Journal | Cellular and molecular life sciences : CMLS
(Cell Mol Life Sci)
Vol. 60
Issue 3
Pg. 567-76
(Mar 2003)
ISSN: 1420-682X [Print] Switzerland |
PMID | 12737316
(Publication Type: Journal Article)
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Chemical References |
- Benzhydryl Compounds
- Carcinogens, Environmental
- Estrogen Receptor beta
- Estrogens, Non-Steroidal
- Phenols
- Receptors, Estrogen
- Estradiol
- 4-tert-octylphenol
- bisphenol A
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Topics |
- Benzhydryl Compounds
- Breast Neoplasms
(metabolism)
- Carcinogens, Environmental
(pharmacology)
- Estradiol
(pharmacology)
- Estrogen Receptor beta
- Estrogens, Non-Steroidal
(pharmacology)
- Female
- Humans
- In Vitro Techniques
- Phenols
(pharmacology)
- Receptors, Estrogen
(drug effects)
- Tumor Cells, Cultured
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