Abstract |
We develop our previous observation that a subpopulation of circulating memory IgM(+) IgD(+)CD27(+)B cells belongs to a separate pathway of differentiation in humans. This subpopulation, which represents 5-25% of peripheral B cells, is also present in spleen in the same proportion and displays a marginal-zone-like B cell phenotype. In addition, we describe a short-time in vitro induction model for somatic hypermutation by using the BL2 Burkitt's lymphoma cell line stimulated by a combination of antibodies directed against different surface receptors. This short-time assay allows us to show that mutations are stably introduced in one DNA strand of the BL2 VH gene in the G1 phase of the cell cycle.
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Authors | Sandra Weller, Ahmad Faili, Said Aoufouchi, Quetin Guéranger, Moritz Braun, Claude-Agnès Reynaud, Jean-Claude Weill |
Journal | Annals of the New York Academy of Sciences
(Ann N Y Acad Sci)
Vol. 987
Pg. 158-65
(Apr 2003)
ISSN: 0077-8923 [Print] United States |
PMID | 12727635
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)
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Chemical References |
- Tumor Necrosis Factor Receptor Superfamily, Member 7
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Topics |
- B-Lymphocytes
(immunology, metabolism)
- Humans
- In Vitro Techniques
- Mutation
- Tumor Necrosis Factor Receptor Superfamily, Member 7
(immunology)
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