Analysis of a shared pancreatic cancer antigen recognized by an HLA-A*2601-restricted cytotoxic T-lymphocyte clone.

Abstract

INTRODUCTION:

We have generated HLA-A*2601-restricted CD8+ CTL clones against an autologous pancreatic cancer cell line.

AIMS:

To characterize the antigen expressed on the cancer cells.

METHODOLOGY:

We assessed cytotoxic activities and cytokine production of these CTL clones reacting against cancer cell lines that stably or transiently expressed the HLA-A*2601 gene.

RESULTS:

These CTL clones recognized 4 of 10 allogeneic pancreatic cancer cell lines and a gallbladder cancer cell line in the context of HLA-A*2601. However, the CTL clones did not recognize three hepatocellular carcinoma cell lines, two esophageal squamous cell carcinoma cell lines, or a lung adenocarcinoma cell line.

CONCLUSIONS:

Thus, the CTL clones may recognize a shared, but not ubiquitously expressed, tumor antigen on pancreatic and gallbladder cancer cells.

• Authors: Kiyoteru Kashiwagi, Hideyuki Ikeda, Yoshihiko Hirohashi, Masayuki Yamamoto, Satomi Idenoue, Itaru Hirai, Kenjiro Kamiguchi, Yasuaki Tamura, Toshiko Torigoe, Yshimasa Wada, Koichi Hirata, Noriyuki Sato
• Journal: Pancreas (Pancreas) Vol. 26 Issue 4 Pg. e81-8 (May 2003) ISSN: 1536-4828 [Electronic] United States
• PMID: 12717278 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• HLA-A Antigens
• HLA-A*26 antigen
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Antigens, Neoplasm (analysis, immunology)
• Cell Line, Tumor
• Cytotoxicity Tests, Immunologic
• Cytotoxicity, Immunologic (immunology)
• Genotype
• Granulocyte-Macrophage Colony-Stimulating Factor (biosynthesis)
• HLA-A Antigens (genetics, immunology)
• Humans
• K562 Cells
• Pancreatic Neoplasms (genetics, immunology, metabolism)
• T-Lymphocytes, Cytotoxic (cytology, immunology)