Abstract | OBJECTIVES: MATERIALS AND METHODS: The material for this investigation of expression of mRNA selected genes was 4 tissue specimens from women with vulvar cancer T1N2M0. The method used for quantitative determination of the numbers of mRNA copies of selected genes was the QRT-PCR and QPCR technique, with the use of an ABI PRISM 7700 (TaqMan) sequence detector. The nucleotide sequence for starters and probes for quantitative RT-PCR was designed with the use of the Primer Express Version 1.0 ABI PRISM software. RESULTS: Expression of all mRNA isoforms VEGF was found in the samples. The number of mRNA copies obtained in 1 microgram total mRNA extract of the genes examined in tissue sections with various pathomorphology were clearly differentiated. CONCLUSIONS:
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Authors | Bogdan Michalski, Urszula Mazurek, Anita Olejek, Tomasz Loch, Michał Graniczka, Ryszard Poreba, Tadeusz Wilczok |
Journal | Ginekologia polska
(Ginekol Pol)
Vol. 74
Issue 1
Pg. 40-7
(Jan 2003)
ISSN: 0017-0011 [Print] Poland |
Vernacular Title | Wzory ekspresji izoform naczyniowo-śródbłonkowego czynnika wzrostu (VEGF) w raku sromu T1N2M0. |
PMID | 12715436
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Endothelial Growth Factors
- Intercellular Signaling Peptides and Proteins
- Lymphokines
- Protein Isoforms
- RNA, Messenger
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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Topics |
- Endothelial Growth Factors
(biosynthesis, genetics)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Intercellular Signaling Peptides and Proteins
(biosynthesis, genetics)
- Lymphokines
(biosynthesis, genetics)
- Neoplasm Staging
- Protein Isoforms
(biosynthesis)
- RNA, Messenger
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
- Vulvar Neoplasms
(chemistry, pathology)
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