HSP70.1 and -70.3 are required for late-phase protection induced by ischemic preconditioning of mouse hearts.

Abstract

We investigated the role of inducible heat shock proteins 70.1 and 70.3 (HSP70.1 and HSP70.3, respectively) in myocardial ischemic preconditioning (IP) in mice. Wild-type (WT) mice and HSP70.1- and HSP70.3-null [HSP70.1/3(-/-)] mice were subjected to IP and examined 24 h later during the late phase of protection. IP significantly increased steady-state levels of HSP70.1 and HSP70.3 mRNA and expression of inducible HSP70 protein in WT myocardium. To assess protection against tissue injury, mice were subjected to 30 min of regional ischemia and 3 h of reperfusion. In WT mice, IP reduced infarct size by 43% compared with sham IP-treated mice. In contrast, IP did not reduce infarct size in HSP70.1/3(-/-) mice. Absence of inducible HSP70.1 and HSP70.3 had no effect, however, on classical or early-phase protection produced by IP, which significantly reduced infarct size in HSP70.1/3(-/-) mice. We conclude that inducible HSP70.1 and HSP70.3 are required for late-phase protection against infarction following IP in mice.

• Authors: Craig R Hampton, Akira Shimamoto, Christine L Rothnie, Jeaneatte Griscavage-Ennis, Albert Chong, David J Dix, Edward D Verrier, Timothy H Pohlman
• Journal: American journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 285 Issue 2 Pg. H866-74 (Aug 2003) ISSN: 0363-6135 [Print] United States
• PMID: 12714332 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• HSP70 Heat-Shock Proteins
• Protozoan Proteins
• RNA, Messenger
• heat-shock protein 70.1

Topics

• Animals
• Cytoplasm (physiology)
• Gene Expression
• HSP70 Heat-Shock Proteins (genetics, metabolism)
• Ischemic Preconditioning, Myocardial
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Myocardial Infarction (metabolism, physiopathology)
• Myocardial Reperfusion Injury (metabolism, physiopathology)
• Protozoan Proteins (genetics, metabolism)
• RNA, Messenger (analysis)