Protection of interferon-gamma knockout mice against Listeria monocytogenes challenge following intramuscular immunization with DNA vaccines encoding listeriolysin O.

In this study we evaluated the efficacy of DNA vaccination of IFN-gamma knockout (GKO) mice against Listeria monocytogenes, as these immunodeficient mice are highly susceptible to infection with low numbers of this intracellular bacterial pathogen. Following intramuscular immunization of BALB/c GKO mice with plasmid DNA constructs encoding recombinant forms of the L. monocytogenes hemolysin, listeriolysin O (LLO), we detected the in vivo induction of a LLO(91-99) peptide-specific, protective immune CTL response equivalent to that observed following similar DNA vaccination of normal BALB/c mice. The observed protection represented greatly enhanced immunity for the GKO host, suggesting that DNA vaccination may provide a useful vaccine alternative for certain immunocompromised host populations.
AuthorsRonald A Barry, H G Archie Bouwer, Tina R Clark, Kenneth A Cornell, David J Hinrichs
JournalVaccine (Vaccine) Vol. 21 Issue 17-18 Pg. 2122-32 (May 16 2003) ISSN: 0264-410X [Print] Netherlands
PMID12706703 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Bacterial Proteins
  • Bacterial Toxins
  • Bacterial Vaccines
  • Heat-Shock Proteins
  • Hemolysin Proteins
  • Peptide Fragments
  • Vaccines, DNA
  • hlyA protein, Listeria monocytogenes
  • Interferon-gamma
  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins (immunology)
  • Bacterial Toxins
  • Bacterial Vaccines (administration & dosage, therapeutic use)
  • Female
  • Heat-Shock Proteins (immunology)
  • Hemolysin Proteins
  • Injections, Intramuscular
  • Interferon-gamma (deficiency, genetics, immunology)
  • Listeria monocytogenes (immunology)
  • Listeriosis (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Peptide Fragments (chemistry, immunology)
  • T-Lymphocytes, Cytotoxic (immunology)
  • Vaccines, DNA (administration & dosage, therapeutic use)

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