| Abstract | In this study we evaluated the efficacy of DNA vaccination of IFN-gamma knockout (GKO) mice against Listeria monocytogenes, as these immunodeficient mice are highly susceptible to infection with low numbers of this intracellular bacterial pathogen. Following intramuscular immunization of BALB/c GKO mice with plasmid DNA constructs encoding recombinant forms of the L. monocytogenes hemolysin, listeriolysin O (LLO), we detected the in vivo induction of a LLO(91-99) peptide-specific, protective immune CTL response equivalent to that observed following similar DNA vaccination of normal BALB/c mice. The observed protection represented greatly enhanced immunity for the GKO host, suggesting that DNA vaccination may provide a useful vaccine alternative for certain immunocompromised host populations. |
| Authors | Ronald A Barry, H G Archie Bouwer, Tina R Clark, Kenneth A Cornell, David J Hinrichs
(Affiliation: Immunology Research, Veterans Affairs Medical Center, P.O. Box 1034, Portland, OR 97207, USA. barryr at ohsu.edu)
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| Journal | Vaccine
(Vaccine)
Vol. 21
Issue 17-18
Pg. 2122-32
(May 16 2003)
ISSN: 0264-410X Netherlands |
| PMID | 12706703
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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| Chemical References |
- Bacterial Proteins
- Bacterial Toxins
- Bacterial Vaccines
- Heat-Shock Proteins
- Hemolysin Proteins
- Peptide Fragments
- Vaccines, DNA
- hlyA protein, Listeria monocytogenes
- Interferon-gamma
|
| Topics |
- Amino Acid Sequence
- Animals
- Bacterial Proteins
(immunology)
- Bacterial Toxins
- Bacterial Vaccines
(administration & dosage, therapeutic use)
- Female
- Heat-Shock Proteins
(immunology)
- Hemolysin Proteins
- Injections, Intramuscular
- Interferon-gamma
(deficiency, genetics, immunology)
- Listeria Infections
(immunology)
- Listeria monocytogenes
(immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
- Peptide Fragments
(chemistry, immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccines, DNA
(administration & dosage, therapeutic use)
|
