Blood culture technique based on centrifugation: clinical evaluation.

Abstract

A total of 1,000 blood samples from patients suspected of having a bacteremia were analyzed concurrently, where possible, by three methods: (i) Trypticase soy broth with sodium polyanethol sulfonate and a CO2 atmosphere: (ii) pour plates with either brain heart infusion agar or Sabouraud dextrose agar; and (iii) centrifugation of the suspected organism in a hypertonic solution. There were 176 positive cultures. The centrifugation technique recovered 73% of the positive cultures. The broth and pour plate techniques recovered 38 and 49%, respectively. The centrifugation technique showed an increased isolation rate for Pseudomonas, fungi, and gram-positive cocci. In general, for each organism the time required for the detection of a positive culture was shortest for the centrifugation technique.

• Authors: G L Dorn, G G Burson, J R Haynes
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 3 Issue 3 Pg. 258-63 (Mar 1976) ISSN: 0095-1137 [Print] United States
• PMID: 1270591 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Hypertonic Solutions

Topics

• Bacteria (isolation & purification)
• Bacterial Infections (microbiology)
• Blood (microbiology)
• Centrifugation, Density Gradient (methods)
• Evaluation Studies as Topic
• Fungi (isolation & purification)
• Humans
• Hypertonic Solutions
• Mycoses (microbiology)
• Species Specificity