HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Potential of lipid core peptide technology as a novel self-adjuvanting vaccine delivery system for multiple different synthetic peptide immunogens.

Abstract
This study demonstrates the effectiveness of a novel self-adjuvanting vaccine delivery system for multiple different synthetic peptide immunogens by use of lipid core peptide (LCP) technology. An LCP formulation incorporating two different protective epitopes of the surface antiphagocytic M protein of group A streptococci (GAS)--the causative agents of rheumatic fever and subsequent rheumatic heart disease--was tested in a murine parenteral immunization and GAS challenge model. Mice were immunized with the LCP-GAS formulation, which contains an M protein amino-terminal type-specific peptide sequence (8830) in combination with a conserved non-host-cross-reactive carboxy-terminal C-region peptide sequence (J8) of the M protein. Our data demonstrated immunogenicity of the LCP-8830-J8 formulation in B10.BR mice when coadministered in complete Freund's adjuvant and in the absence of a conventional adjuvant. In both cases, immunization led to induction of high-titer GAS peptide-specific serum immunoglobulin G antibody responses and induction of highly opsonic antibodies that did not cross-react with human heart tissue proteins. Moreover, mice were completely protected from GAS infection when immunized with LCP-8830-J8 in the presence or absence of a conventional adjuvant. Mice were not protected, however, following immunization with an LCP formulation containing a control peptide from a Schistosoma sp. These data support the potential of LCP technology in the development of novel self-adjuvanting multi-antigen component vaccines and point to the potential application of this system in the development of human vaccines against infectious diseases.
AuthorsColleen Olive, Michael Batzloff, Aniko Horváth, Timothy Clair, Penny Yarwood, Istvan Toth, Michael F Good
JournalInfection and immunity (Infect Immun) Vol. 71 Issue 5 Pg. 2373-83 (May 2003) ISSN: 0019-9567 [Print] United States
PMID12704107 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Carrier Proteins
  • Immunoglobulin G
  • Lipids
  • Peptide Fragments
  • Streptococcal Vaccines
  • Vaccines, Synthetic
  • streptococcal M protein
Topics
  • Amino Acid Sequence
  • Animals
  • Antibodies, Bacterial (blood)
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins (immunology)
  • Carrier Proteins (immunology)
  • Cross Reactions
  • Drug Delivery Systems
  • Female
  • Immunoglobulin G (blood, classification)
  • Lipids
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments (immunology)
  • Phagocytosis
  • Streptococcal Vaccines (administration & dosage, immunology)
  • Streptococcus pyogenes (immunology)
  • Vaccines, Synthetic (administration & dosage)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: