Abstract |
The ability to vaccinate against polyomavirus infection in a T-cell deficient as well as a normal immune context was studied using polyomavirus major capsid protein (VP1) pseudocapsids (VP1-ps) or a glutathione-S-transferase-VP1 (GST-VP1) fusion protein. VP1-ps (1 or 10 microg) were administered subcutaneously, alone or together with Freund's complete and incomplete adjuvant, to CD4(-/-)8(-/-) T-cell deficient or normal C57Bl/6 mice on four occasions. Alternatively, CD4(-/-)8(-/-) and normal mice were inoculated with either GST-VP1 or Py-VP1-ps (5 microg). Following immunisation, antibody titres were tested by ELISA to VP1-ps or GST-VP1 or by haemagglutination inhibition (HAI). Mice were then infected with polyomavirus. Three weeks post- infection, the mice were killed and examined for the presence of polyomavirus DNA by PCR. Viral DNA was not detected in CD4(-/-)8(-/-) mice immunised with either VP1-ps alone or in combination with Freund's complete and incomplete adjuvant, or in any of the normal mice immunised with VP1-ps or GST-VP1. However, viral DNA was detected in 2/5 of the CD4(-/-)8(-/-) mice immunised with GST-VP1 and in non-immunised controls. Greater antibody titres were observed to VP1-ps than to GST-VP1 in CD4(-/-)8(-/-) mice after VP1-ps compared to GST-VP1 immunisation and antibody responses were better in normal than in immune-deficient mice. Only immunisation with VP1-ps resulted in haemagglutination inhibition. Complete protection against polyomavirus infection in the T-cell deficient context was obtained with VP1-ps, but not with GST-VP1, immunisation using the present vaccination protocol.
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Authors | Andrea Vlastos, Kalle Andreasson, Karin Tegerstedt, Dana Holländerová, Shirin Heidari, Jitka Forstová, Torbjörn Ramqvist, Tina Dalianis |
Journal | Journal of medical virology
(J Med Virol)
Vol. 70
Issue 2
Pg. 293-300
(Jun 2003)
ISSN: 0146-6615 [Print] United States |
PMID | 12696121
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2003 Wiley-Liss, Inc. |
Chemical References |
- Antibodies, Viral
- Capsid Proteins
- Recombinant Fusion Proteins
- VP1 protein, polyomavirus
- Viral Vaccines
- Glutathione Transferase
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Topics |
- Animals
- Antibodies, Viral
(blood)
- Capsid
- Capsid Proteins
(genetics, immunology, metabolism)
- Disease Models, Animal
- Glutathione Transferase
(genetics, immunology, metabolism)
- Guinea Pigs
- Hemagglutination
- Humans
- Immunization
- Immunologic Deficiency Syndromes
(immunology)
- Mice
- Mice, Inbred C57BL
- Polyomavirus
(immunology)
- Polyomavirus Infections
(prevention & control, virology)
- Recombinant Fusion Proteins
(immunology)
- T-Lymphocytes
(immunology)
- Tumor Virus Infections
(prevention & control, virology)
- Viral Vaccines
(administration & dosage, immunology)
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