Abstract |
A method is described for increasing the sensitivity of conventional counterimmunoelectrophoresis (CIEP), i.e. double sequential counterimmunoelectrophoresis (DCIEP). Our method consists of a second electrophoretic migration made after the classical CIEP, where instead of HB antibody (from polytransfused patients), the anodic wells are filled with an antihuman-gammaglobulin antiserum. DCIEP is significantly more sensitive than CIEP (P less than 0.01), the number of positive sera in chronic lever diseases being 28.7% compared with 17.5%.
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Authors | O Abrudan, D Dejica |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 69
Issue 1
Pg. 113-5
(May 17 1976)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 1269147
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Chronic Disease
- Counterimmunoelectrophoresis
(methods)
- Hepatitis B Antigens
(analysis)
- Humans
- Liver Diseases
(diagnosis, immunology)
- Microchemistry
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