The differential effects of chemical carcinogens on vitamin A status and on microsomal drug metabolism in normal and vitamin A-deficient rats.

Abstract

Male Sprague-Dawley rats were maintained on a vitamin A-deficient diet for 5 weeks. Although serum and hepatic levels of vitamin A were significantly lower at this time, no outward signs of vitamin A deficiency were present. Hepatic microsomal levels of cytochrome P-450 in the vitamin A-deficient animals were 70% that of the control animals. Of the three microsomal enzymes studied, ethylmorphine N-demethylase, aniline hydroxylase, and aminopyrine N-demethylase, only the last one was adversely affected by vitamin A deficiency. 3-Methylcholanthrene, phenobarbital, and 2-acetylaminofluorene had a greater inductive effect and cytochrome P-450 in vitamin A-deficient rats. 4-Dimethylaminoazobenzene treatment decreased in the level of cytochrome P-450 in control rats more than in deficieny rats. The hepatic concentration of vitamin A was significantly reduced in control rats that were given injections of 3-methylcholanthrene, 2-acetylaminofluorene, or phenobarbital. Benzo(a)pyrene and 4-dimethylaminoazobenzene had less effect.

• Authors: J W Hauswirth, B S Brizuela
• Journal: Cancer research (Cancer Res) Vol. 36 Issue 6 Pg. 1941-6 (Jun 1976) ISSN: 0008-5472 [Print] United States
• PMID: 1268849 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Aniline Compounds
• Carcinogens
• Aminopyrine
• Vitamin A
• Morphine
• Cytochrome P-450 Enzyme System
• Aniline Hydroxylase
• Aminopyrine N-Demethylase
• Ethylmorphine-N-Demethylase

Topics

• Aminopyrine (metabolism)
• Aminopyrine N-Demethylase (metabolism)
• Aniline Compounds (metabolism)
• Aniline Hydroxylase (metabolism)
• Animals
• Body Weight
• Carcinogens (pharmacology)
• Cytochrome P-450 Enzyme System (metabolism)
• Ethylmorphine-N-Demethylase (metabolism)
• Liver (metabolism)
• Male
• Microsomes, Liver (enzymology, metabolism)
• Morphine (metabolism)
• Organ Size
• Rats
• Vitamin A (blood, metabolism)
• Vitamin A Deficiency (metabolism)