The effect of poly (adenosine diphosphate-ribose) polymerase inhibitors on biochemical changes in testicular ischemia-reperfusion injury.

Poly (adenosine diphosphate [ADP]-ribose) polymerase inhibitors have been used successfully to decrease ischemia-reperfusion injury in several organ systems. We evaluated the efficacy of poly (ADP-ribose) polymerase inhibitors on biochemical changes in testicular ischemia-reperfusion injury.
Adult male Wistar rats were divided into 9 groups of 6 each. One group served to determine baseline values of biochemical parameters, 1 that underwent sham operation served as a control, 1 underwent 2 hours of testicular torsion and 4 hours of detorsion, 2 received pretreatment with vehicle (saline or dimethyl sulfoxide) before detorsion and 4 received pretreatment with the poly (ADP-ribose) polymerase inhibitor nicotinamide, 3-aminobenzamide, 1,5-dihydroxyisoquinoline or 4-amino-1,8-naphthalimide before detorsion. Lipid peroxidation products, nitric oxide content and myeloperoxidase activity, an indicator of neutrophil accumulation, were assessed in testicular and renal tissues.
Testicular torsion-detorsion caused a significant increase in lipid peroxidation products, nitric oxide content and myeloperoxidase activity in ipsilateral testes (p <0.01) but not in the contralateral testes or kidneys. Animals treated with poly (ADP-ribose) polymerase inhibitors had a significant decrease in these biochemical parameters compared with vehicle treated animals (p <0.01).
These data emphasize that poly (ADP-ribose) polymerase may have a role in testicular damage caused by ischemia-reperfusion and the inhibition of poly (ADP-ribose) polymerase may be a novel approach to therapy for ischemia-reperfusion injury of the testis.
AuthorsMurat Bozlu, Gülçin Eskandari, Selahittin Cayan, Bülent Canpolat, Erdem Akbay, Uğur Atik
JournalThe Journal of urology (J Urol) Vol. 169 Issue 5 Pg. 1870-3 (May 2003) ISSN: 0022-5347 [Print] United States
PMID12686864 (Publication Type: Journal Article)
Chemical References
  • Benzamides
  • Isoquinolines
  • Naphthalimides
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Quinolones
  • 4-amino-1,8-naphthalimide
  • Niacinamide
  • 1,5-dihydroxyisoquinoline
  • 3-aminobenzamide
  • 1-Naphthylamine
  • 1-Naphthylamine (analogs & derivatives, therapeutic use)
  • Animals
  • Benzamides (therapeutic use)
  • Isoquinolines (therapeutic use)
  • Male
  • Naphthalimides
  • Niacinamide (therapeutic use)
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Quinolones (therapeutic use)
  • Rats
  • Rats, Wistar
  • Reperfusion Injury (drug therapy, enzymology, etiology)
  • Spermatic Cord Torsion (complications, drug therapy, enzymology)
  • Testis (blood supply, drug effects)

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