Similar diagnostic performance for neurocysticercosis of three glycoprotein preparations from Taenia solium metacestodes.

Abstract

The detection of antibodies to Taenia solium metacestodes is very important in the differential diagnosis of neurocysticercosis (NCC). In this study, an electroimmunotransfer blot (EITB) assay that uses an elaborate protocol with metacestode glycoproteins as antigens was compared with two other Western blots that use glycoproteins obtained using simpler methods, including an eluate from a lectin column, or the vesicular fluid (VF) of the parasite. The concordance between the three assays was 91% in patients with active NCC and 100% in patients with suspected NCC and previous documentation of negative serology. The specificities for the Western blots and the EITB assay were 98% and 100%, respectively (98% concordance). These data suggest that the simplest of these immunoassays, the one that uses the VF of T. solium metacestodes in a Western blot format, can be reliably used for the serologic diagnosis of NCC in developing countries where access to the EITB assay is difficult.

• Authors: Guido E Villota, Diana I Gomez, Michel Volcy, Andrés F Franco, Edgar A Cardona, Rodrigo Isaza, Fernando Sanzón, Judy M Teale, Blanca I Restrepo
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 68 Issue 3 Pg. 276-80 (Mar 2003) ISSN: 0002-9637 [Print] United States
• PMID: 12685629 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Glycoproteins
• Helminth Proteins

Topics

• Blotting, Western
• Glycoproteins
• Helminth Proteins
• Humans
• Molecular Sequence Data
• Neurocysticercosis (diagnosis, parasitology)
• Sensitivity and Specificity
• Taenia solium (metabolism)