Effects of lecithinized superoxide dismutase on neuronal cell loss in CA3 hippocampus after traumatic brain injury in rats.

Abstract

BACKGROUND:

The protective effect of excitatory amino acid antagonists for CA3 hippocampal neuronal loss has been well documentated. From a clinical point of view, however, alternative therapies should also be explored because excitatory amino acid antagonists have relatively deleterious side effects. Administration of lecithinized superoxide dismutase (PC-SOD) has recently been demonstrated to reduce brain edema after traumatic brain injury (TBI) in the cerebral cortex. In this study, we investigated the effectiveness of PC-SOD on CA3 hippocampal cell loss by examining hematoxylin and eosin-stained sections.

METHODS:

Rats were divided at random into three groups. The first group received 1 mL of saline (contusion + saline group, n = 5). Rats of the second group were treated with 3000 IU/kg of PC-SOD (contusion + SOD 1 group, n = 5), while the third group received 5000 IU/kg of PC-SOD (contusion + SOD 2 group, n = 5). All agents were administered intraperitoneally 1 minute after traumatic insult and every 24 hours until 2 or 3 days post-TBI. Animals were sacrificed 3 or 7 days after contusion injury.

RESULTS:

PC-SOD prevented CA3 neuronal loss 3 days after TBI, and increased the number of surviving CA3 neurons 7 days after TBI.

CONCLUSION:

Our findings suggest that PC-SOD may serve as a pharmacological agent in the treatment of neuronal loss after TBI.

• Authors: Masatoshi Yunoki, Masamitsu Kawauchi, Naoya Ukita, Tomoyuki Sugiura, Takashi Ohmoto
• Journal: Surgical neurology (Surg Neurol) Vol. 59 Issue 3 Pg. 156-60; discussion 160-1 (Mar 2003) ISSN: 0090-3019 [Print] United States
• PMID: 12681536 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphatidylcholines
• Superoxide Dismutase
• lecithinized superoxide dismutase

Topics

• Animals
• Brain Injuries (complications, drug therapy, pathology)
• Cell Count
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Administration Schedule
• Hippocampus (drug effects, injuries, pathology)
• Male
• Nerve Degeneration (etiology, pathology, prevention & control)
• Phosphatidylcholines (administration & dosage, therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (administration & dosage, therapeutic use)
• Time Factors