Abstract | INTRODUCTION: The overall haemostatic potential (OHP) assay records fibrin polymerisation in plasma by areas under the opacity curve. The authors reported high area values in pregnancy, and still higher values in preeclampsia. We wanted to see if the assay detects thrombophilia, and particularly, if the area values were high in congenital combined protein S (PS) and the FV R506Q Leiden mutation, since low protein S and increased activated protein C ( APC) resistance occur in pregnancy and preeclampsia. MATERIALS AND METHODS: RESULTS: In the original assay, median area values in controls and thrombophilic plasma samples were similar. In the modified assay, area values tended to be lower in controls than in the thrombophilia group (P=0.035). The enhanced inhibition reduced area values more in control plasmas (median reduction 34.7%) than in thrombophilic plasmas (median reduction 2.2%) (P=0.0017). The responses to activation were also low in warfarin-treated patients with thrombophilia. CONCLUSIONS: High area values in pregnancy with the original assay are probably not caused by insufficient inhibition. The response to activation of protein C and antithrombin, as calculated by the difference between the original and a modified assay, was subnormal in thrombophilic plasma, but there was an overlap with the results in controls.
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Authors | Marianne S Andresen, Nina Iversen, Ulrich Abildgaard |
Journal | Thrombosis research
(Thromb Res)
Vol. 108
Issue 5-6
Pg. 323-8
(Dec 15 2002)
ISSN: 0049-3848 [Print] United States |
PMID | 12676193
(Publication Type: Journal Article)
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Chemical References |
- Protein C
- factor V Leiden
- Warfarin
- Antithrombin III
- Factor V
- Fibrin
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Topics |
- Adult
- Aged
- Antithrombin III
(physiology)
- Area Under Curve
- Blood Coagulation Tests
(methods)
- Case-Control Studies
- Factor V
- Family Health
- Female
- Fibrin
(metabolism)
- Hemostasis
- Humans
- Male
- Middle Aged
- Pregnancy
- Protein C
(physiology)
- Protein S Deficiency
- Thrombophilia
(blood, genetics)
- Warfarin
(pharmacology)
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