Tyrosine-kinase expression profiles in human gastric cancer cell lines and their modulations with retinoic acids.

Abstract

Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways. Obtaining a comprehensive tyrosine-kinase expression profile in tumour cells is essential to learning more about their oncogenic potentials and responses to various chemotherapeutic reagents - such as retinoic acid, which has been shown to suppress the growth of gastric cancer cells and modulate gene expression. Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique. We first established comprehensive tyrosine-kinase profiles in different human gastric cancer cell lines. In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated. In the present study, we demonstrate an efficient and simple RAGE approach for examining tyrosine kinases' expression in tumour cells and their alterations following drug treatments.

• Authors: H-W Kao, H-C Chen, C-W Wu, W-C Lin
• Journal: British journal of cancer (Br J Cancer) Vol. 88 Issue 7 Pg. 1058-64 (Apr 07 2003) ISSN: 0007-0920 [Print] England
• PMID: 12671705 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RARA protein, human
• Receptors, Retinoic Acid
• Retinoic Acid Receptor alpha
• Tretinoin
• Protein-Tyrosine Kinases
• Receptor, EphA3
• Receptors, Eph Family

Topics

• Gene Expression Profiling
• Humans
• Protein-Tyrosine Kinases (genetics)
• Receptor, EphA3 (genetics)
• Receptors, Eph Family (genetics)
• Receptors, Retinoic Acid (physiology)
• Retinoic Acid Receptor alpha
• Stomach Neoplasms (enzymology)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured
• Retinoic Acid Receptor gamma