An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease.

Abstract

In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.

• Authors: Min Zhu, Mingfa Wei, Fang Liu
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 22 Issue 2 Pg. 152-4 ( 2002) ISSN: 1672-0733 [Print] China
• PMID: 12658761 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Heme Oxygenase (Decyclizing)
• heme oxygenase-2

Topics

• Child
• Child, Preschool
• Colon (enzymology)
• Female
• Heme Oxygenase (Decyclizing) (biosynthesis, genetics)
• Hirschsprung Disease (enzymology, etiology, genetics)
• Humans
• Infant
• Infant, Newborn
• Male
• RNA, Messenger (biosynthesis, genetics)