Abstract | PURPOSE: METHODS: RESULTS: Compared with the control, increased accumulations of type I and type III collagens were detected by immunohistochemistry in fibrotic conjunctiva of patients with OCP. Weak and sparse expression of HSP47 was detected in the epithelial cells and stromal fibroblasts in control conjunctival tissues. In contrast to the control, the expression of HSP47 was markedly increased in the stromal fibroblasts in conjunctival tissues obtained from patients with OCP, as detected by immunohistochemistry. By quantitative real-time PCR, compared with control conjunctival tissues, a 3.4-fold increase in the expression of HSP47 was noted in the conjunctival tissues obtained from patients with OCP. Similar to conjunctival tissues, fibroblasts isolated from conjunctiva of patients with OCP exhibited 4.8-fold increase in the expression of HSP47, compared with control fibroblasts. When conjunctival fibroblasts were treated with various concentration of TGF-beta1, upregulation in the expression of HSP47 and type I collagen was detected. CONCLUSIONS: This study demonstrated increased expression of HSP47 and TGF-beta1 by conjunctival fibroblasts in biopsy specimens obtained from patients with OCP. TGF-beta1 induced the expression of HSP47 and type I collagen by conjunctival fibroblasts. Increased levels of TGF-beta1 and HSP47 may regulate increased synthesis, assembly, and production of collagens and thereby could significantly contribute to the process of conjunctival scarring in patients with OCP.
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Authors | Mohammed S Razzaque, C Stephen Foster, A Razzaque Ahmed |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 44
Issue 4
Pg. 1616-21
(Apr 2003)
ISSN: 0146-0404 [Print] United States |
PMID | 12657600
(Publication Type: Journal Article)
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Chemical References |
- Collagen Type I
- Collagen Type III
- HSP47 Heat-Shock Proteins
- Heat-Shock Proteins
- RNA, Messenger
- SERPINH1 protein, human
- TGFB1 protein, human
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
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Topics |
- Blotting, Western
- Collagen Type I
(metabolism)
- Collagen Type III
(metabolism)
- Conjunctiva
(cytology, drug effects, metabolism)
- Conjunctivitis
(metabolism, pathology)
- Fibroblasts
(drug effects, metabolism)
- HSP47 Heat-Shock Proteins
- Heat-Shock Proteins
(genetics, metabolism)
- Humans
- Immunoenzyme Techniques
- Pemphigoid, Benign Mucous Membrane
(metabolism, pathology)
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transforming Growth Factor beta
(metabolism, pharmacology)
- Transforming Growth Factor beta1
- Up-Regulation
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