Abstract | OBJECTIVE: Growth patterns of astrocytic tumors can be modulated in vitro by gastrin. In this study, the influence of gastrin on the in vitro cell cycle kinetics and the in vivo growth features of three experimental malignant gliomas was investigated. METHODS:
Gastrin-induced influence on overall growth was assayed in vitro by means of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium colorimetric assay for human U373 and rat C6 gliomas and for rat 9L gliosarcoma. Although cell cycle analyses were performed by means of computer-assisted microscope analyses of Feulgen-stained nuclei, the gastrin-induced effects of the levels of expression of cyclins D3 and E, CDK2, CDK4, CDK5, CDK7, p15, p16, E2F1, and E2F2 were assayed by means of quantitative Western blot test. The presence of ribonucleic acids for the CCK(B) and CCK(C) gastrin receptors in the U373, C6, and 9L models was assayed by means of quantitative reverse transcriptase-polymerase chain reaction, and the presence or absence of ribonucleic acids for CCK(A) receptor was checked by means of conventional polymerase chain reaction. The influence of gastrin was also characterized in vivo in terms of the survival periods of conventional rats orthotopically grafted with the C6 and 9L models and nude rats with the U373 model. RESULTS: CONCLUSION:
Gastrin is able to significantly modify the growth levels of a number of experimental gliomas.
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Authors | Florence Lefranc, Niloufar Sadeghi, Thierry Metens, Jacques Brotchi, Isabelle Salmon, Robert Kiss |
Journal | Neurosurgery
(Neurosurgery)
Vol. 52
Issue 4
Pg. 881-90; discussion 890-1
(Apr 2003)
ISSN: 0148-396X [Print] United States |
PMID | 12657185
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCK-C receptor
- Cyclins
- Gastrins
- Protein Isoforms
- Receptor, Cholecystokinin A
- Receptors, Cholecystokinin
- Protein Kinases
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Topics |
- Animals
- Brain Neoplasms
(pathology)
- Cell Cycle
(drug effects)
- Cell Division
(drug effects)
- Cyclins
(genetics)
- Gastrins
(pharmacology)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glioma
(pathology)
- Gliosarcoma
(pathology)
- Humans
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence
- Polymerase Chain Reaction
- Protein Isoforms
(genetics)
- Protein Kinases
(genetics)
- Rats
- Receptor, Cholecystokinin A
- Receptors, Cholecystokinin
(drug effects)
- Tumor Cells, Cultured
(drug effects, pathology)
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