A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.

Abstract

In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).

• Authors: Peter Lawonn, Else K Hoffmann, Charlotte Hougaard, Frank Wehner
• Journal: FEBS letters (FEBS Lett) Vol. 539 Issue 1-3 Pg. 115-9 (Mar 27 2003) ISSN: 0014-5793 [Print] England
• PMID: 12650937 (Publication Type: Journal Article)

Chemical References

• Cations
• Ion Channels

Topics

• Animals
• Carcinoma, Ehrlich Tumor (metabolism)
• Cations
• Cell Size
• Electric Conductivity
• Ion Channels (drug effects, metabolism)
• Patch-Clamp Techniques
• Tumor Cells, Cultured