[Intranasal immunization against dental caries with plasmid DNA encoding pac gene of Streptococcus mutans in gnotobiotic rats].

Abstract

OBJECTIVES:

To assess the efficacy of plasmid DNA encoding pac gene of Streptococcus mutans (S. mutans) intranasally immunized in gnotobiotic rats and to compare the effect of two different delivery systems.

METHODS:

Sprague Dawley rats, infected with S. mutans at 20 days of age, were intranasally immunized with plasmid pCIA-P (group A), Dosper-DNA complex (group B), Bupivacaine-DNA complex (group C). Control rats were either immunized with plasmid pCI (group D), distilled water (group E) or immunized intramuscularly (group F). All the rats were boosted 2 weeks later. ELISA determined the antibodies against the vaccines. Keyes caries score was used to evaluate the anti- caries effectiveness of the vaccines at the terminal study.

RESULTS:

As for the antibody reactions, there were significantly (P < 0.01) differences between rats immunized with DNA vaccine and non-immunized rats. And rats in group B and C had the significantly (P < 0.01) higher level of specific salivary anti-PAc IgA antibodies and rats (group B, C, F) had the significantly (P < 0.01) higher specific serum anti-PAc IgG responses to DNA vaccine. Keyes scores of rats (group B and C) were significantly (P < 0.01) lower than others.

CONCLUSIONS:

Intranasal immunization with plasmid pCIA-P encoding pac gene successfully reduces the caries and appears to be a promising approach against dental caries. Cationic liposome Dosper and local anesthetic bupivacaine could enhance the efficacy of DNA vaccine.

• Authors: Jihua Guo, Mingwen Fan, Zhuan Bian, Rong Jia, Bin Peng
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 37 Issue 6 Pg. 452-5 (Nov 2002) ISSN: 1002-0098 [Print] China
• PMID: 12641962 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Bacterial Proteins
• Immunoglobulin A
• Immunoglobulin G
• Membrane Glycoproteins
• S-layer proteins
• Streptococcal Vaccines
• Vaccines, DNA

Topics

• Administration, Intranasal
• Animals
• Antibodies, Bacterial (blood)
• Bacterial Proteins (genetics)
• Dental Caries (prevention & control)
• Female
• Immunization
• Immunoglobulin A (blood)
• Immunoglobulin G (blood)
• Membrane Glycoproteins
• Plasmids (genetics)
• Rats
• Rats, Sprague-Dawley
• Streptococcal Vaccines (genetics, immunology, therapeutic use)
• Streptococcus mutans (genetics, immunology)
• Treatment Outcome
• Vaccines, DNA (genetics, immunology, therapeutic use)