| Abstract | Changes in the biological efficacy of leptin were evaluated in obesity-resistant (A/J) and obesity-prone (C57BL/6J) mice at weaning and after consuming a high-fat (HF) diet for 4 and 8 wk. There was no evidence of leptin resistance in either strain at the start of the study, but after 4 and 8 wk on the HF diet, C57BL/6J mice became unresponsive to ip leptin. C57BL/6J mice responded to intracerebroventricular leptin at these time points but developed peripheral resistance to sympathetic stimulation of retroperitoneal white adipose tissue. In contrast, intracerebroventricular leptin was fully effective in A/J mice, reproducing the complete profile of responses observed in weanling mice. A/J mice were also partially responsive to ip leptin at both time points, increasing uncoupling protein 1 mRNA expression in brown adipose tissue and decreasing leptin mRNA in white adipose tissue. The findings indicate that retention of leptin responsiveness is an important component of the ability of A/J mice to mount a robust adaptive thermogenic response and resist diet-induced obesity. |
| Authors | Veronica Prpic, Patricia M Watson, Isabell C Frampton, Mark A Sabol, G Eric Jezek, Thomas W Gettys
(Affiliation: Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808, USA.)
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| Journal | Endocrinology
(Endocrinology)
Vol. 144
Issue 4
Pg. 1155-63
(Apr 2003)
ISSN: 0013-7227 United States |
| PMID | 12639896
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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| Chemical References |
- Carrier Proteins
- DNA-Binding Proteins
- Dietary Fats
- Ion Channels
- Leptin
- Membrane Proteins
- Mitochondrial Proteins
- STAT3 Transcription Factor
- Stat3 protein, mouse
- Trans-Activators
- mitochondrial uncoupling protein
|
| Topics |
- Adipose Tissue
(drug effects, metabolism)
- Animals
- Carrier Proteins
(genetics)
- DNA-Binding Proteins
(metabolism)
- Dietary Fats
(pharmacology)
- Energy Intake
- Gene Expression
(drug effects, physiology)
- Hypothalamus
(drug effects, metabolism)
- Ion Channels
- Leptin
(metabolism, pharmacology)
- Membrane Proteins
(genetics)
- Mice
- Mice, Inbred A
- Mice, Inbred C57BL
- Mitochondrial Proteins
- Obesity
(metabolism)
- STAT3 Transcription Factor
- Species Specificity
- Trans-Activators
(metabolism)
|
