Enhanced vascular production of superoxide in OLETF rat after the onset of hyperglycemia.

Abstract	This study was aimed to characterize the vascular production of superoxide in Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model of type 2 diabetes. The nitroblue tetrazolium staining in the aorta from old (30 weeks) OLETF rat was more prominent than that of age-matched control (LETO) rat, which was significantly inhibited by diphenyleneiodonium (10 micromol/l), but not by inhibitors for other oxidases such as xanthine oxidase, mitochondrial oxidase, nitric oxide synthase, and cyclooxygenase. In the aorta from old OLETF rat with hyperglycemia, the enhanced NADH oxidase activity in association with upregulated expression of p22phox and gp91phox was observed, but not in both LETO and young (10 weeks) OLETF rats without hyperglycemia. Furthermore, there was a positive correlation (P<0.01) between elevation of blood glucose level and increase in vascular NADH oxidase activity. Based on these results, it was suggested that the enhanced NADH oxidase activity in the aorta from OLETF rat occurred after the onset of hyperglycemia, thereby resulting in the increased vascular production of superoxide.
Authors	In J Kim, Yong K Kim, Seok M Son, Ki W Hong, Chi D Kim
Journal	Diabetes research and clinical practice (Diabetes Res Clin Pract) Vol. 60 Issue 1 Pg. 11-8 (Apr 2003) ISSN: 0168-8227 [Print] Ireland
PMID	12639760 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Membrane Glycoproteins Membrane Transport Proteins Multienzyme Complexes Phosphoproteins Superoxides NADH oxidase NADH, NADPH Oxidoreductases CYBB protein, human NADPH Oxidase 2 NADPH Oxidases CYBA protein, human NADPH Dehydrogenase
Topics	Aging Animals Aorta (enzymology, metabolism) Diabetes Mellitus, Type 2 (metabolism) Disease Models, Animal Hyperglycemia (metabolism) Male Membrane Glycoproteins (metabolism) Membrane Transport Proteins Multienzyme Complexes (metabolism) Muscle, Smooth, Vascular (enzymology, metabolism) NADH, NADPH Oxidoreductases (metabolism) NADPH Dehydrogenase (metabolism) NADPH Oxidase 2 NADPH Oxidases Phosphoproteins (metabolism) Rats Rats, Inbred OLETF Reference Values Superoxides (metabolism)

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