Abstract |
Immunomodulatory activity of an Ayurvedic polyherbal formulation, Immu-21 containing extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia was studied on proliferative response of splenic leukocytes to T cell mitogens, concanavalin (Con)-A and phytohemagglutinin (PHA) and B cell mitogen, lipopolysaccharide (LPS) in vitro by [3H]- thymidine uptake assay in mice. The cytotoxic activity of Immu-21 was tested by measuring the splenic leukocyte natural killer (NK) cell activity against K 562 cells. Intraperitoneal (i.p.) treatment with Immu-21 (30 mg/kg) once a day for 14 and 21 days did not cause change in body weight and spleen weight, where as splenocytes/spleen count was increased. Treatment of Immu-21 (30 mg/kg, i.p.) for 14 days and 1 mg/kg for 21 days significantly increased LPS induced leukocyte proliferation. NK cell activity was significantly increased when mice were pretreated with Immu-21 (10 and 30 mg/kg, i.p.) once a day for 7 days. The results indicate that pretreatment with Immu-21 selectively increased the proliferation of splenic leukocyte to B cell mitogen, LPS and cytotoxic activity against K 562 cells in mice.
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Authors | Kumar V S Nemmani, G B Jena, C S Dey, C L Kaul, P Ramarao |
Journal | Indian journal of experimental biology
(Indian J Exp Biol)
Vol. 40
Issue 3
Pg. 282-7
(Mar 2002)
ISSN: 0019-5189 [Print] India |
PMID | 12635697
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Plant Extracts
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- B-Lymphocytes
(drug effects, immunology)
- Cytotoxicity, Immunologic
(drug effects)
- Humans
- K562 Cells
- Killer Cells, Natural
(drug effects, immunology)
- Lymphocyte Activation
(drug effects)
- Medicine, Ayurvedic
- Plant Extracts
(pharmacology)
- Plants, Medicinal
- T-Lymphocytes
(drug effects, immunology)
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