Abstract |
Current detection of transmissible spongiform encephalopathy (TSE) relies on the proteolytic generation of a protease-resistant core from the scrapie isoform of prion protein (PrP(Sc)) followed by immunoblotting. This process is non-quantitative, time-consuming, and technically demanding. Recently, an alternative in vitro test for TSE based on the differential extraction of brain homogenates using guanidine hydrochloride followed by DELFIA (Dissociation Enhanced Lanthanide FluoroImmunoAssay) has been developed. In the present study, this approach was adopted using a panel of anti-PrP monoclonal antibodies (MAbs) in conventional sandwich enzyme-linked immunosorbent assay (ELISA) to investigate hamster and two distinct strains of mouse prion diseases. Although PrP species were present in both soluble and insoluble fractions from normal as well as TSE samples, only the PrP species in the insoluble fractions from the latter samples were protease-resistant. In addition, certain anti-PrP MAb pairs could distinguish the PrP species in infected brains from those in the normal samples. The ability to differentiate disease-associated PrP isoforms without proteinase K digestion could serve as a panacea for developing a reliable and rapid diagnostic test for prion diseases.
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Authors | Shin-Chung Kang, Ruliang Li, Chuanping Wang, Tao Pan, Tong Liu, Richard Rubenstein, Geoff Barnard, Boon-Seng Wong, Man-Sun Sy |
Journal | The Journal of pathology
(J Pathol)
Vol. 199
Issue 4
Pg. 534-41
(Apr 2003)
ISSN: 0022-3417 [Print] England |
PMID | 12635145
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2003 John Wiley & Sons, Ltd. |
Chemical References |
- Antibodies, Monoclonal
- PrPSc Proteins
- Prions
- Guanidine
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Brain Chemistry
- Cricetinae
- Enzyme-Linked Immunosorbent Assay
(methods)
- Guanidine
- Mesocricetus
- Mice
- Mice, Inbred Strains
- PrPSc Proteins
(analysis, immunology, isolation & purification)
- Prion Diseases
(diagnosis)
- Prions
(analysis, immunology, isolation & purification)
- Solubility
- Specimen Handling
(methods)
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